fluoromount g mounting medium with dapi Search Results


dapi fluoromount g mounting medium  (SouthernBiotech)
96
SouthernBiotech dapi fluoromount g mounting medium
Dapi Fluoromount G Mounting Medium, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dapi fluoromountg mounting medium  (SouthernBiotech)
97
SouthernBiotech dapi fluoromountg mounting medium
Fig. 8 Expression patterns of selected genes. A–F Expression patterns of named gene products or derivatives in germaria co-stained with <t>DAPI</t> (blue in A–D) to reveal all nuclei and antibody to Fas3 (red in A–D; white in E–G) to allow location of the anterior edge of strong Fas3 expression (yellow arrows). A–A” Semaphorin 1a-GFP protein fusion (green) was detected on the surface of somatic cells; levels were low in region 1 (extreme anterior, left), peaked in region 2a ECs, FSCs, and early FCs, and declined in region 3 (posterior of the germarium) and the first budded egg chamber. B–B” Dystroglycan, detected by an antibody to the C-terminus (green), which connects the plasma membrane to ECM components, was seen lining the germarial wall from region 2a (white arrowheads in B’) and continuing posterior, consistent with its expected role and expression in all FSCs, early FCs, and a limited number of the most posterior ECs. Expression was also seen in the muscle sheath (cyan arrowheads in B). C–C” A Plum-GFP protein fusion (green) encoded by a large BAC genomic transgene was expressed most prominently in somatic cells, with highest levels in the FSC region and further posterior. D–D” A Cyclin B-GFP protein fusion showed occasional expression in layer1 FSCs (white arrow in D’ and D”) immediately anterior to the Fas3 border and was seen at similar levels in most FCs. Absence in some FCs is most likely due to protein degradation in G1 and S phases. Expression at the anterior of the germarium is within germline cells. E–G Santa Maria-Gal4 > UAS-RFP produced no RFP signal in most germaria. In other germaria, few cells expressed detectable RFP, including germaria with RFP signal in E a layer 1 FSC (white arrow) and a neighboring FC, F, G an FC adjacent to the Fas3 border, and G another early FC anterior to region 3. All bars, 20 µm
Dapi Fluoromountg Mounting Medium, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dapi fluoromountg mounting medium/product/SouthernBiotech
Average 97 stars, based on 1 article reviews
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fluoromount-g™-dapi  (Biozol Diagnostica Vertrieb GmbH)
90
Biozol Diagnostica Vertrieb GmbH fluoromount-g™-dapi
Fig. 8 Expression patterns of selected genes. A–F Expression patterns of named gene products or derivatives in germaria co-stained with <t>DAPI</t> (blue in A–D) to reveal all nuclei and antibody to Fas3 (red in A–D; white in E–G) to allow location of the anterior edge of strong Fas3 expression (yellow arrows). A–A” Semaphorin 1a-GFP protein fusion (green) was detected on the surface of somatic cells; levels were low in region 1 (extreme anterior, left), peaked in region 2a ECs, FSCs, and early FCs, and declined in region 3 (posterior of the germarium) and the first budded egg chamber. B–B” Dystroglycan, detected by an antibody to the C-terminus (green), which connects the plasma membrane to ECM components, was seen lining the germarial wall from region 2a (white arrowheads in B’) and continuing posterior, consistent with its expected role and expression in all FSCs, early FCs, and a limited number of the most posterior ECs. Expression was also seen in the muscle sheath (cyan arrowheads in B). C–C” A Plum-GFP protein fusion (green) encoded by a large BAC genomic transgene was expressed most prominently in somatic cells, with highest levels in the FSC region and further posterior. D–D” A Cyclin B-GFP protein fusion showed occasional expression in layer1 FSCs (white arrow in D’ and D”) immediately anterior to the Fas3 border and was seen at similar levels in most FCs. Absence in some FCs is most likely due to protein degradation in G1 and S phases. Expression at the anterior of the germarium is within germline cells. E–G Santa Maria-Gal4 > UAS-RFP produced no RFP signal in most germaria. In other germaria, few cells expressed detectable RFP, including germaria with RFP signal in E a layer 1 FSC (white arrow) and a neighboring FC, F, G an FC adjacent to the Fas3 border, and G another early FC anterior to region 3. All bars, 20 µm
Fluoromount G™ Dapi, supplied by Biozol Diagnostica Vertrieb GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dapi fluoromount-g  (Cambridge Bioscience)
90
Cambridge Bioscience dapi fluoromount-g
Fig. 8 Expression patterns of selected genes. A–F Expression patterns of named gene products or derivatives in germaria co-stained with <t>DAPI</t> (blue in A–D) to reveal all nuclei and antibody to Fas3 (red in A–D; white in E–G) to allow location of the anterior edge of strong Fas3 expression (yellow arrows). A–A” Semaphorin 1a-GFP protein fusion (green) was detected on the surface of somatic cells; levels were low in region 1 (extreme anterior, left), peaked in region 2a ECs, FSCs, and early FCs, and declined in region 3 (posterior of the germarium) and the first budded egg chamber. B–B” Dystroglycan, detected by an antibody to the C-terminus (green), which connects the plasma membrane to ECM components, was seen lining the germarial wall from region 2a (white arrowheads in B’) and continuing posterior, consistent with its expected role and expression in all FSCs, early FCs, and a limited number of the most posterior ECs. Expression was also seen in the muscle sheath (cyan arrowheads in B). C–C” A Plum-GFP protein fusion (green) encoded by a large BAC genomic transgene was expressed most prominently in somatic cells, with highest levels in the FSC region and further posterior. D–D” A Cyclin B-GFP protein fusion showed occasional expression in layer1 FSCs (white arrow in D’ and D”) immediately anterior to the Fas3 border and was seen at similar levels in most FCs. Absence in some FCs is most likely due to protein degradation in G1 and S phases. Expression at the anterior of the germarium is within germline cells. E–G Santa Maria-Gal4 > UAS-RFP produced no RFP signal in most germaria. In other germaria, few cells expressed detectable RFP, including germaria with RFP signal in E a layer 1 FSC (white arrow) and a neighboring FC, F, G an FC adjacent to the Fas3 border, and G another early FC anterior to region 3. All bars, 20 µm
Dapi Fluoromount G, supplied by Cambridge Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dapi fluoromount-g mounting medium  (SouthernBiotech)
90
SouthernBiotech dapi fluoromount-g mounting medium
Fig. 8 Expression patterns of selected genes. A–F Expression patterns of named gene products or derivatives in germaria co-stained with <t>DAPI</t> (blue in A–D) to reveal all nuclei and antibody to Fas3 (red in A–D; white in E–G) to allow location of the anterior edge of strong Fas3 expression (yellow arrows). A–A” Semaphorin 1a-GFP protein fusion (green) was detected on the surface of somatic cells; levels were low in region 1 (extreme anterior, left), peaked in region 2a ECs, FSCs, and early FCs, and declined in region 3 (posterior of the germarium) and the first budded egg chamber. B–B” Dystroglycan, detected by an antibody to the C-terminus (green), which connects the plasma membrane to ECM components, was seen lining the germarial wall from region 2a (white arrowheads in B’) and continuing posterior, consistent with its expected role and expression in all FSCs, early FCs, and a limited number of the most posterior ECs. Expression was also seen in the muscle sheath (cyan arrowheads in B). C–C” A Plum-GFP protein fusion (green) encoded by a large BAC genomic transgene was expressed most prominently in somatic cells, with highest levels in the FSC region and further posterior. D–D” A Cyclin B-GFP protein fusion showed occasional expression in layer1 FSCs (white arrow in D’ and D”) immediately anterior to the Fas3 border and was seen at similar levels in most FCs. Absence in some FCs is most likely due to protein degradation in G1 and S phases. Expression at the anterior of the germarium is within germline cells. E–G Santa Maria-Gal4 > UAS-RFP produced no RFP signal in most germaria. In other germaria, few cells expressed detectable RFP, including germaria with RFP signal in E a layer 1 FSC (white arrow) and a neighboring FC, F, G an FC adjacent to the Fas3 border, and G another early FC anterior to region 3. All bars, 20 µm
Dapi Fluoromount G Mounting Medium, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dapi  (Thermo Fisher)
90
Thermo Fisher dapi
Fig. 8 Expression patterns of selected genes. A–F Expression patterns of named gene products or derivatives in germaria co-stained with <t>DAPI</t> (blue in A–D) to reveal all nuclei and antibody to Fas3 (red in A–D; white in E–G) to allow location of the anterior edge of strong Fas3 expression (yellow arrows). A–A” Semaphorin 1a-GFP protein fusion (green) was detected on the surface of somatic cells; levels were low in region 1 (extreme anterior, left), peaked in region 2a ECs, FSCs, and early FCs, and declined in region 3 (posterior of the germarium) and the first budded egg chamber. B–B” Dystroglycan, detected by an antibody to the C-terminus (green), which connects the plasma membrane to ECM components, was seen lining the germarial wall from region 2a (white arrowheads in B’) and continuing posterior, consistent with its expected role and expression in all FSCs, early FCs, and a limited number of the most posterior ECs. Expression was also seen in the muscle sheath (cyan arrowheads in B). C–C” A Plum-GFP protein fusion (green) encoded by a large BAC genomic transgene was expressed most prominently in somatic cells, with highest levels in the FSC region and further posterior. D–D” A Cyclin B-GFP protein fusion showed occasional expression in layer1 FSCs (white arrow in D’ and D”) immediately anterior to the Fas3 border and was seen at similar levels in most FCs. Absence in some FCs is most likely due to protein degradation in G1 and S phases. Expression at the anterior of the germarium is within germline cells. E–G Santa Maria-Gal4 > UAS-RFP produced no RFP signal in most germaria. In other germaria, few cells expressed detectable RFP, including germaria with RFP signal in E a layer 1 FSC (white arrow) and a neighboring FC, F, G an FC adjacent to the Fas3 border, and G another early FC anterior to region 3. All bars, 20 µm
Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dapi-fluoromount gtm  (Thermo Fisher)
90
Thermo Fisher dapi-fluoromount gtm
Fig. 8 Expression patterns of selected genes. A–F Expression patterns of named gene products or derivatives in germaria co-stained with <t>DAPI</t> (blue in A–D) to reveal all nuclei and antibody to Fas3 (red in A–D; white in E–G) to allow location of the anterior edge of strong Fas3 expression (yellow arrows). A–A” Semaphorin 1a-GFP protein fusion (green) was detected on the surface of somatic cells; levels were low in region 1 (extreme anterior, left), peaked in region 2a ECs, FSCs, and early FCs, and declined in region 3 (posterior of the germarium) and the first budded egg chamber. B–B” Dystroglycan, detected by an antibody to the C-terminus (green), which connects the plasma membrane to ECM components, was seen lining the germarial wall from region 2a (white arrowheads in B’) and continuing posterior, consistent with its expected role and expression in all FSCs, early FCs, and a limited number of the most posterior ECs. Expression was also seen in the muscle sheath (cyan arrowheads in B). C–C” A Plum-GFP protein fusion (green) encoded by a large BAC genomic transgene was expressed most prominently in somatic cells, with highest levels in the FSC region and further posterior. D–D” A Cyclin B-GFP protein fusion showed occasional expression in layer1 FSCs (white arrow in D’ and D”) immediately anterior to the Fas3 border and was seen at similar levels in most FCs. Absence in some FCs is most likely due to protein degradation in G1 and S phases. Expression at the anterior of the germarium is within germline cells. E–G Santa Maria-Gal4 > UAS-RFP produced no RFP signal in most germaria. In other germaria, few cells expressed detectable RFP, including germaria with RFP signal in E a layer 1 FSC (white arrow) and a neighboring FC, F, G an FC adjacent to the Fas3 border, and G another early FC anterior to region 3. All bars, 20 µm
Dapi Fluoromount Gtm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluoromount dapi  (Millipore)
90
Millipore fluoromount dapi
Fig. 8 Expression patterns of selected genes. A–F Expression patterns of named gene products or derivatives in germaria co-stained with <t>DAPI</t> (blue in A–D) to reveal all nuclei and antibody to Fas3 (red in A–D; white in E–G) to allow location of the anterior edge of strong Fas3 expression (yellow arrows). A–A” Semaphorin 1a-GFP protein fusion (green) was detected on the surface of somatic cells; levels were low in region 1 (extreme anterior, left), peaked in region 2a ECs, FSCs, and early FCs, and declined in region 3 (posterior of the germarium) and the first budded egg chamber. B–B” Dystroglycan, detected by an antibody to the C-terminus (green), which connects the plasma membrane to ECM components, was seen lining the germarial wall from region 2a (white arrowheads in B’) and continuing posterior, consistent with its expected role and expression in all FSCs, early FCs, and a limited number of the most posterior ECs. Expression was also seen in the muscle sheath (cyan arrowheads in B). C–C” A Plum-GFP protein fusion (green) encoded by a large BAC genomic transgene was expressed most prominently in somatic cells, with highest levels in the FSC region and further posterior. D–D” A Cyclin B-GFP protein fusion showed occasional expression in layer1 FSCs (white arrow in D’ and D”) immediately anterior to the Fas3 border and was seen at similar levels in most FCs. Absence in some FCs is most likely due to protein degradation in G1 and S phases. Expression at the anterior of the germarium is within germline cells. E–G Santa Maria-Gal4 > UAS-RFP produced no RFP signal in most germaria. In other germaria, few cells expressed detectable RFP, including germaria with RFP signal in E a layer 1 FSC (white arrow) and a neighboring FC, F, G an FC adjacent to the Fas3 border, and G another early FC anterior to region 3. All bars, 20 µm
Fluoromount Dapi, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4 6 diamidino 2 phenylindole  (Thermo Fisher)
86
Thermo Fisher 4 6 diamidino 2 phenylindole
Fig. 8 Expression patterns of selected genes. A–F Expression patterns of named gene products or derivatives in germaria co-stained with <t>DAPI</t> (blue in A–D) to reveal all nuclei and antibody to Fas3 (red in A–D; white in E–G) to allow location of the anterior edge of strong Fas3 expression (yellow arrows). A–A” Semaphorin 1a-GFP protein fusion (green) was detected on the surface of somatic cells; levels were low in region 1 (extreme anterior, left), peaked in region 2a ECs, FSCs, and early FCs, and declined in region 3 (posterior of the germarium) and the first budded egg chamber. B–B” Dystroglycan, detected by an antibody to the C-terminus (green), which connects the plasma membrane to ECM components, was seen lining the germarial wall from region 2a (white arrowheads in B’) and continuing posterior, consistent with its expected role and expression in all FSCs, early FCs, and a limited number of the most posterior ECs. Expression was also seen in the muscle sheath (cyan arrowheads in B). C–C” A Plum-GFP protein fusion (green) encoded by a large BAC genomic transgene was expressed most prominently in somatic cells, with highest levels in the FSC region and further posterior. D–D” A Cyclin B-GFP protein fusion showed occasional expression in layer1 FSCs (white arrow in D’ and D”) immediately anterior to the Fas3 border and was seen at similar levels in most FCs. Absence in some FCs is most likely due to protein degradation in G1 and S phases. Expression at the anterior of the germarium is within germline cells. E–G Santa Maria-Gal4 > UAS-RFP produced no RFP signal in most germaria. In other germaria, few cells expressed detectable RFP, including germaria with RFP signal in E a layer 1 FSC (white arrow) and a neighboring FC, F, G an FC adjacent to the Fas3 border, and G another early FC anterior to region 3. All bars, 20 µm
4 6 Diamidino 2 Phenylindole, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluoromont-gtm medium  (Thermo Fisher)
90
Thermo Fisher fluoromont-gtm medium
Fig. 8 Expression patterns of selected genes. A–F Expression patterns of named gene products or derivatives in germaria co-stained with <t>DAPI</t> (blue in A–D) to reveal all nuclei and antibody to Fas3 (red in A–D; white in E–G) to allow location of the anterior edge of strong Fas3 expression (yellow arrows). A–A” Semaphorin 1a-GFP protein fusion (green) was detected on the surface of somatic cells; levels were low in region 1 (extreme anterior, left), peaked in region 2a ECs, FSCs, and early FCs, and declined in region 3 (posterior of the germarium) and the first budded egg chamber. B–B” Dystroglycan, detected by an antibody to the C-terminus (green), which connects the plasma membrane to ECM components, was seen lining the germarial wall from region 2a (white arrowheads in B’) and continuing posterior, consistent with its expected role and expression in all FSCs, early FCs, and a limited number of the most posterior ECs. Expression was also seen in the muscle sheath (cyan arrowheads in B). C–C” A Plum-GFP protein fusion (green) encoded by a large BAC genomic transgene was expressed most prominently in somatic cells, with highest levels in the FSC region and further posterior. D–D” A Cyclin B-GFP protein fusion showed occasional expression in layer1 FSCs (white arrow in D’ and D”) immediately anterior to the Fas3 border and was seen at similar levels in most FCs. Absence in some FCs is most likely due to protein degradation in G1 and S phases. Expression at the anterior of the germarium is within germline cells. E–G Santa Maria-Gal4 > UAS-RFP produced no RFP signal in most germaria. In other germaria, few cells expressed detectable RFP, including germaria with RFP signal in E a layer 1 FSC (white arrow) and a neighboring FC, F, G an FC adjacent to the Fas3 border, and G another early FC anterior to region 3. All bars, 20 µm
Fluoromont Gtm Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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medium with dapi  (Thermo Fisher)
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Thermo Fisher medium with dapi
Fig. 8 Expression patterns of selected genes. A–F Expression patterns of named gene products or derivatives in germaria co-stained with <t>DAPI</t> (blue in A–D) to reveal all nuclei and antibody to Fas3 (red in A–D; white in E–G) to allow location of the anterior edge of strong Fas3 expression (yellow arrows). A–A” Semaphorin 1a-GFP protein fusion (green) was detected on the surface of somatic cells; levels were low in region 1 (extreme anterior, left), peaked in region 2a ECs, FSCs, and early FCs, and declined in region 3 (posterior of the germarium) and the first budded egg chamber. B–B” Dystroglycan, detected by an antibody to the C-terminus (green), which connects the plasma membrane to ECM components, was seen lining the germarial wall from region 2a (white arrowheads in B’) and continuing posterior, consistent with its expected role and expression in all FSCs, early FCs, and a limited number of the most posterior ECs. Expression was also seen in the muscle sheath (cyan arrowheads in B). C–C” A Plum-GFP protein fusion (green) encoded by a large BAC genomic transgene was expressed most prominently in somatic cells, with highest levels in the FSC region and further posterior. D–D” A Cyclin B-GFP protein fusion showed occasional expression in layer1 FSCs (white arrow in D’ and D”) immediately anterior to the Fas3 border and was seen at similar levels in most FCs. Absence in some FCs is most likely due to protein degradation in G1 and S phases. Expression at the anterior of the germarium is within germline cells. E–G Santa Maria-Gal4 > UAS-RFP produced no RFP signal in most germaria. In other germaria, few cells expressed detectable RFP, including germaria with RFP signal in E a layer 1 FSC (white arrow) and a neighboring FC, F, G an FC adjacent to the Fas3 border, and G another early FC anterior to region 3. All bars, 20 µm
Medium With Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluoromount g dapi  (Thermo Fisher)
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Thermo Fisher fluoromount g dapi
Fig. 8 Expression patterns of selected genes. A–F Expression patterns of named gene products or derivatives in germaria co-stained with <t>DAPI</t> (blue in A–D) to reveal all nuclei and antibody to Fas3 (red in A–D; white in E–G) to allow location of the anterior edge of strong Fas3 expression (yellow arrows). A–A” Semaphorin 1a-GFP protein fusion (green) was detected on the surface of somatic cells; levels were low in region 1 (extreme anterior, left), peaked in region 2a ECs, FSCs, and early FCs, and declined in region 3 (posterior of the germarium) and the first budded egg chamber. B–B” Dystroglycan, detected by an antibody to the C-terminus (green), which connects the plasma membrane to ECM components, was seen lining the germarial wall from region 2a (white arrowheads in B’) and continuing posterior, consistent with its expected role and expression in all FSCs, early FCs, and a limited number of the most posterior ECs. Expression was also seen in the muscle sheath (cyan arrowheads in B). C–C” A Plum-GFP protein fusion (green) encoded by a large BAC genomic transgene was expressed most prominently in somatic cells, with highest levels in the FSC region and further posterior. D–D” A Cyclin B-GFP protein fusion showed occasional expression in layer1 FSCs (white arrow in D’ and D”) immediately anterior to the Fas3 border and was seen at similar levels in most FCs. Absence in some FCs is most likely due to protein degradation in G1 and S phases. Expression at the anterior of the germarium is within germline cells. E–G Santa Maria-Gal4 > UAS-RFP produced no RFP signal in most germaria. In other germaria, few cells expressed detectable RFP, including germaria with RFP signal in E a layer 1 FSC (white arrow) and a neighboring FC, F, G an FC adjacent to the Fas3 border, and G another early FC anterior to region 3. All bars, 20 µm
Fluoromount G Dapi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 8 Expression patterns of selected genes. A–F Expression patterns of named gene products or derivatives in germaria co-stained with DAPI (blue in A–D) to reveal all nuclei and antibody to Fas3 (red in A–D; white in E–G) to allow location of the anterior edge of strong Fas3 expression (yellow arrows). A–A” Semaphorin 1a-GFP protein fusion (green) was detected on the surface of somatic cells; levels were low in region 1 (extreme anterior, left), peaked in region 2a ECs, FSCs, and early FCs, and declined in region 3 (posterior of the germarium) and the first budded egg chamber. B–B” Dystroglycan, detected by an antibody to the C-terminus (green), which connects the plasma membrane to ECM components, was seen lining the germarial wall from region 2a (white arrowheads in B’) and continuing posterior, consistent with its expected role and expression in all FSCs, early FCs, and a limited number of the most posterior ECs. Expression was also seen in the muscle sheath (cyan arrowheads in B). C–C” A Plum-GFP protein fusion (green) encoded by a large BAC genomic transgene was expressed most prominently in somatic cells, with highest levels in the FSC region and further posterior. D–D” A Cyclin B-GFP protein fusion showed occasional expression in layer1 FSCs (white arrow in D’ and D”) immediately anterior to the Fas3 border and was seen at similar levels in most FCs. Absence in some FCs is most likely due to protein degradation in G1 and S phases. Expression at the anterior of the germarium is within germline cells. E–G Santa Maria-Gal4 > UAS-RFP produced no RFP signal in most germaria. In other germaria, few cells expressed detectable RFP, including germaria with RFP signal in E a layer 1 FSC (white arrow) and a neighboring FC, F, G an FC adjacent to the Fas3 border, and G another early FC anterior to region 3. All bars, 20 µm

Journal: BMC biology

Article Title: Single-cell expression profile of Drosophila ovarian follicle stem cells illuminates spatial differentiation in the germarium.

doi: 10.1186/s12915-023-01636-9

Figure Lengend Snippet: Fig. 8 Expression patterns of selected genes. A–F Expression patterns of named gene products or derivatives in germaria co-stained with DAPI (blue in A–D) to reveal all nuclei and antibody to Fas3 (red in A–D; white in E–G) to allow location of the anterior edge of strong Fas3 expression (yellow arrows). A–A” Semaphorin 1a-GFP protein fusion (green) was detected on the surface of somatic cells; levels were low in region 1 (extreme anterior, left), peaked in region 2a ECs, FSCs, and early FCs, and declined in region 3 (posterior of the germarium) and the first budded egg chamber. B–B” Dystroglycan, detected by an antibody to the C-terminus (green), which connects the plasma membrane to ECM components, was seen lining the germarial wall from region 2a (white arrowheads in B’) and continuing posterior, consistent with its expected role and expression in all FSCs, early FCs, and a limited number of the most posterior ECs. Expression was also seen in the muscle sheath (cyan arrowheads in B). C–C” A Plum-GFP protein fusion (green) encoded by a large BAC genomic transgene was expressed most prominently in somatic cells, with highest levels in the FSC region and further posterior. D–D” A Cyclin B-GFP protein fusion showed occasional expression in layer1 FSCs (white arrow in D’ and D”) immediately anterior to the Fas3 border and was seen at similar levels in most FCs. Absence in some FCs is most likely due to protein degradation in G1 and S phases. Expression at the anterior of the germarium is within germline cells. E–G Santa Maria-Gal4 > UAS-RFP produced no RFP signal in most germaria. In other germaria, few cells expressed detectable RFP, including germaria with RFP signal in E a layer 1 FSC (white arrow) and a neighboring FC, F, G an FC adjacent to the Fas3 border, and G another early FC anterior to region 3. All bars, 20 µm

Article Snippet: Each ovary pair was then broken apart in PBS on a slide and mounted using DAPI fluoromountG mounting medium (Southern BioTech, OB010020).

Techniques: Expressing, Staining, Clinical Proteomics, Membrane, Produced